proviral integration site meaning in Chinese

前簿整合位点,原簿整合位点

The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element , containing receptor binding sites and major antigenic determinants . exjsrv - specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals , validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛随机引物法标记exjsrv特异的env片段，制备探针，原位杂交检测spa肺组织中的rna及前病毒dna ，结果表明spa患羊肺组织内有jsrvenv基因mrna的表达，同时也检测到了前病毒dna ，而相应的阴性对照却无阳性信号，证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。